HISTOLOGICAL AND HISTOCHEMICAL CHANGES OF THYMIC NURSE CELLS AND IMMUNE ORGANS OF MOUSE AFTER THE INOCULATION OF LEUKEMIC CELLS / 解剖学报

It was discovered in this experiment that 3 days after inoculation of the ascitic fluid from P_(388) leukemic mice, the positive percentages of AcP and ?-Gase of the lymphocytes within the TNC of the recipients were increased significantly, while the positive percentage of ANAE was decreased. All the positive percentages of the three enzymes of the thymocytes within the thymus were increased significantly, but no abnormal structural changes were seen in the thymus. No histological and cytochemical changes were found in the lymph nodes and the spleen. Nine days after inoculation, the leukemia cell infiltration, hemorrhage and cell degeneration occurred in all the thymus, lymph nodes and spleen of the mouse. The normal structures of the organs were destroyed. The positive percentages of the cytochemical reactions of the three enzymes in the lymphocytes were all increased significantly. At this stage, TNC was very difficult to be isolated from the thymus. The results indicate that thymus and TNC may have a close relationship with the development of this type of leukemia.

STEREOLOGIC ANALYSIS OF THE LYMPHO CYTES LOCATED AT INTRA-AND EXTRAFOLLICULAR DENDRITIC CELL DISTRIBUTION AREA / 解剖学报

HRP-anti-HRP complex was injected into BALB/c mice through the tail vein. 24 hours later, the spleens of mice were removed, fixed and cut into slices. After incubation in DAB/H_2O_2 the follicular dendritic cell (FDC) distribution area could be defined at a certain part of lymph nodule. Light microscopic and stereologic analysis study on the lymphocytes at intra- and extra- FDC distribution area were carried out. The results revealed that lymphocytes at intra- and extra- FDC distribution area were composed of various lymphocytes populations of different sizes. The cell populations at intra-FDC distribution area were mainly composed of large and medium sized lymphocytes, while the populations at extra-FDC distribution area were mainly medium sized and small lymphocytes. As a result, FDC always exists on the location where lymphoblastic phenomenon appears. This indicates that FDC has certain relation with the lymphoblastic phenomenon.

THE ULTRASTRUCTURAL OBSERVATIONS ON THE CHANGES OF FOLLICULAR DENDRITIC CELLS IN THE SPLEEN OF THE POSTNATAL RATS AT THE AGES OF 21 TO 48 DAYS / 解剖学报

In this experiment, the PAP was used as a specific marker to label the follicular dendritic cells (FDC) and their possible precursors in the spleens of postnatal rats at 21, 27, 34, 41 and 48 days respectively, and the changes of the labeled cells were studied under TEM. In 21-day-old rats the labeled cells were of the histological feature resembling fibroblastic reticulum cells(FRC). As the rats getting elder, the shape and structure of the labeled cells changed. The shape of t he cells and nuclei changed from ellipse to irregular. The heterochromatin decreased and so did the microfilaments in the cytoplasm. The cytoplasmic processes increased in number, length and complexity. The relatively smooth cell surface became folding and invaginating. By the 48 days after birth the labeled cells possessed the features of the FDC. The result implys that the FDC might originate from FRC.